IMAC metal chelate affinity chromatography resins
Immobilized metal affinity chromatography (IMAC) resin is designed to purify a wide range of proteins. It is supplied free of metal ions(uncharged), allowing the user to charge it with the most appropriate metal ion for optimized selectivity. The resin selectively retains proteins based on the specific interaction between certain amino acid side chains exposed on the surface of proteins (mainly histidine and to a lesser extent cysteine and tryptophan) and transition metal ions, most often Zn2+, Ni2+, Cu2+ or Co2+.
